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AssigneesPhiladelphia Health and Education Corporation, University of Medicine and Dentistry of New Jersey
InventorsIra B. Black, Dale L. Woodbury, Darwin J. Prockop, Emily Schwarz
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The present invention relates to methods of inducing differentiation of mammalian bone marrow stromal cells into neuronal cells by contacting marrow stromal cells with a neuronal differentiation-inducing compounds. Neuronal differentiation-inducing compounds of the invention include anti-oxidants such as, but not limited to, beta-mercaptoethanol, dimethylsulfoxide, butylated hydroxyanisole, butylated hydroxytoluene, ascorbic acid, dimethylfumarate, and n-acetylcysteine. Once induced to differentiate into neuronal cells, the cells can be used for cell therapy, gene therapy, or both, for treatment of diseases, disorders, or conditions of the central nervous system.
An in vitro method of inducing differentiation of an isolated marrow stromal cell into a neuronal cell, said method comprising contacting said isolated marrow stromal cell with a neuronal differentiation-inducing compound, wherein said neuronal differentiation-inducing compound is an anti-oxidant, thereby inducing differentiation of said isolated marrow stromal cell into a neuronal cell in vitro.
An in vitro method of producing an isolated neuronal cell, said method comprising isolating a marrow stromal cell, contacting said marrow stromal cell with a neuronal differentiation-inducing compound, wherein said neuronal differentiation-inducing compound is an anti-oxidant, wherein said compound induces said isolated marrow stromal cell to differentiate into an isolated neuronal cell, thereby producing said isolated neuronal cell in vitro.
An isolated neuronal cell made by an in vitro method of inducing differentiation of an isolated marrow stromal cell, said method comprising contacting said isolated marrow stromal cell with at least one neuronal differentiation-inducing compound in vitro, wherein said neuronal differentiation-inducing compound is an anti-oxidant, thereby inducing differentiation of said isolated marrow stromal cell into said neuronal cell.
An isolated neuronal cell made by an in vitro method of producing an isolated neuronal cell, said method comprising isolating a marrow stromal cell, contacting said marrow stromal cell with a neuronal differentiation inducing compound, wherein said neuronal differentiation-inducing compound is an anti-oxidant, further wherein said compound induces said isolated marrow stromal cell to differentiate into said isolated neuronal cell, thereby producing said isolated neuronal cell in vitro.
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