US 7,279,105 B2 · Arrangement and method for performing chromatography

Abstract

An arrangement for performing chromatography with a mobile phase and a chromatographic bed having a stationary phase is provided. The arrangement includes a support assembly configured to support the stationary phase. The support assembly has a sealed cavity configured so that when the support assembly is supporting the stationary phase a portion of the chromatographic bed and the stationary phase extends into the sealed cavity. The arrangement further includes a bladder having a void defined therein. The bladder is positioned within the sealed cavity. The arrangement also includes a fluid positioned within the void of the bladder so that the bladder is urged toward the stationary phase within the sealed cavity. The arrangement further includes an anode positioned in contact with the mobile phase. The arrangement also includes a cathode positioned in contact with the stationary phase, wherein creating an electrical potential between the anode and the cathode causes the mobile phase to be advanced through the chromatographic bed positioned within the sealed cavity. An associated method is also provided.

Claims12

Dependent claims

A method of performing chromatography with a chromatographic bed having a stationary phase, comprising the steps of:

positioning the chromatographic bed and the stationary phase within a sealed cavity;
placing the stationary phase in contact with a liquid mobile phase while the stationary phase is positioned within the sealed cavity;
advancing a fluid into the sealed cavity so that pressure within the sealed cavity is greater than the pressure outside of the sealed cavity;
placing a first electrode in contact with the liquid mobile phase;
placing a second electrode in contact with the chromatographic bed;
and
creating an electrical potential between the first electrode and the second electrode so as to cause the liquid mobile phase to be advanced through the chromatographic bed positioned within the sealed cavity.

4 more elements

Dependencies are inferred from the claim text.

Arrangement and method for performing chromatography

Life of this patent

Arrangement and method for performing chromatography

Life of this patent

Abstract

Claims12