Isolated genomic polynucleotide fragments from chromosome 10q25.3 that encode human soluble aminopeptidase P
AssigneeRyogen LLC
InventorJames W. Ryan
Life of this patent
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Abstract
The invention is directed to an isolated genomic polynucleotide fragment that encodes human soluble (cytosolic) aminopeptidase P, vectors and hosts containing the fragment and fragments hybridizing to noncoding regions as well as antisense oligonucleotides to these fragments. The invention is further directed to methods of using these fragments to obtain human soluble aminopeptidase P and to diagnose, treat, prevent and/or ameliorate a pathological disorder.
Claims21
An isolated genomic polynucleotide, wherein said polynucleotide can be isolated from human chromosome 10 and is selected from the group consisting of:
- (a) a polynucleotide consisting of the nucleic acid sequence set forth in SEQ ID NO:2,
- (b) a polynucleotide consisting of the sequence of nucleotides from position 12803 to position 55760 of SEQ ID NO:2, and
- (c) a polynucleotide which is the complement of a polynucleotide of (a) or (b) and encodes a polypeptide having aminoacyl prolyl peptidyl hydrolase activity.
An expression vector comprising a polynucleotide consisting of the sequence of nucleotides from position 12803 to position 55760 of SEQ ID NO:2 or the complement thereof in operable linkage with a heterologous promoter.
A method of identifying a nucleotide sequence variant of SEQ ID NO:2 or its complementary sequence comprising
- (a) isolating genomic DNA from a subject, and
- (b) determining the presence or absence of a nucleotide sequence variation in said genomic DNA by comparing the nucleotide sequence of SEQ ID NO:2 with the nucleotide sequence of the isolated genomic DNA and establishing if and where a difference occurs between the two nucleotide sequences thereby identifying a nucleotide sequence variant of SEQ ID NO:2.
An isolated polynucleotide consisting of a nucleic acid sequence selected from the group consisting of
- (a) a sequence of at least 5000 contiguous nucleotides within the region consisting of the sequence of nucleotides from position 1 through position 12802 of SEQ ID NO:2,
- (b) a sequence of at least 2000 contiguous nucleotides within the region consisting of the sequence of nucleotides from position 55761 through position 58735 of SEQ ID NO:2,
- (c) a sequence of nucleotides complementary to the nucleotide sequence of (a), and
- (d) a sequence of nucleotides complementary to the nucleotide sequence of (b).
Dependencies are inferred from the claim text.