# US 7,273,617 B2 · Infectious cDNA clones of porcine reproductive and respiratory syndrome virus and expression vectors thereof

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- Number: US 7,273,617 B2
- Document ID: US7273617B2
- Status: Granted
- Kind: B2
- Application number: 10/977,375
- Filed: 2004-10-29
- Published: 2005-10-27
- Granted: 2007-09-25
- Earliest claimed: 2002-01-22
- Assignees: ProtaTek International, Inc.
- Inventors: Shishan Yuan; Shi-jun Ma
- Web page: https://patentgrep.com/patent/US7273617B2

## CPC

- C07K 14/005: from viruses
- A61K 39/12: Viral antigens
- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- A61K 39/00: Medicinal preparations containing antigens or antibodies (materials for immunoassay G01N33/53)
- A61K 2039/552: {Veterinary vaccine}
- C12N 2770/10021: Viruses as such, e.g. new isolates, mutants or their genomic sequences
- C12N 2770/10022: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- C12N 2770/10034: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Infectious cDNA clones of North American Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) are provided. Further provided are cDNA clones comprising genetic mutations. Also provided are vaccines comprising cDNAs, including genetically and immunologically marked vaccines for North American PRRSV.

## Claims

### Claim 1

An isolated polynucleotide comprising the nucleotide sequence of SEQ ID NO: 1 or a degenerate variant of SEQ ID NO: 1.

### Claim 2 (depends on 1)

A vector comprising the polynucleotide of claim 1.

### Claim 3

An isolated polynucleotide comprising a modified nucleotide sequence of SEQ ID NO:1, wherein the modified nucleotide sequence comprises a marker sequence.

### Claim 4 (depends on 3)

The isolated polynucleotide of claim 3, wherein the marker sequence comprises a restriction enzyme recognition sequence or a polynucleotide sequence that encodes an immunologic polypeptide.

### Claim 5 (depends on 4)

The isolated polynucleotide of claim 4, wherein the marker sequence encodes a porcine viral polypeptide.

### Claim 6 (depends on 5)

The isolated polynucleotide of claim 5, wherein the porcine viral polypeptide comprises a capsid protein of Porcine Circovirus Type II or a hemagglutinin protein of Swine Influenza virus.

### Claim 7 (depends on 3)

The isolated polynucleotide of claim 3, wherein the marker sequence comprises at least one foreign polynucleotide sequence inserted:
(a) between nucleotide positions 12181-12182 of SEQ ID NO: 1; or
(b) between nucleotide positions 15382-15383 of SEQ ID NO: 1.

### Claim 8 (depends on 3)

The isolated polynucleotide of claim 3, comprising a substitution at nucleotide position 14305 and position 14307 of SEQ ID NO: 1.

### Claim 9 (depends on 3)

The isolated polynucleotide of claim 3, wherein at least one nucleotide is deleted:
(a) between nucleotide positions 15364-15370 of SEQ ID NO: 1; or
(b) between nucleotide positions 15371-15375 of SEQ ID NO: 1.