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AssigneeBASF SE
InventorsMarkus Pompejus, Burkhard Kröger, Hartwig Schröder, Oskar Zelder, Gregor Haberhauer
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Isolated nucleic acid molecules, designated sugar metabolism and oxidative phosphorylation (SMP) nucleic acid molecules, which encode novel SMP proteins from Corynebacterium glutamicum, are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing SMP nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated SMP proteins, mutated SMP proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. glutamicum based on genetic engineering of SMP genes in this organism.
An isolated nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:1, or the complement thereof, wherein said nucleic acid molecule encodes a polypeptide having 6-phosphogluconolactonase activity and wherein said nucleic acid molecule comprises less than 5 kb of nucleotide sequences which naturally flank the nucleotide sequence of SEQ ID NO:1.
An isolated nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:1, or the complement thereof, wherein said nucleic acid molecule encodes only a polypeptide having 6-phosphogluconolactonase activity.
An isolated nucleic acid molecule comprising a nucleotide sequence which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2, or the complement thereof, wherein the polypeptide has 6-phosphogluconolactonase activity and wherein said nucleic acid molecule comprises less than 5 kb of nucleotide sequences which naturally flank the nucleotide sequence encoding the amino acid sequence of SEQ ID NO:2.
An isolated nucleic acid molecule comprising a nucleotide sequence which has at least 95% identity with the nucleotide sequence of SEQ ID NO:1, or the complement thereof, wherein said nucleic acid molecule encodes a polypeptide having 6-phosphogluconolactonase activity and wherein said nucleic acid molecule comprises less than 5 kb of nucleotide sequences which naturally flank the nucleotide sequence of SEQ ID NO:1.
An isolated nucleic acid molecule which encodes only a polypeptide comprising the amino acid sequence of SEQ ID NO:2 and having 6-phosphogluconolactonase activity, or the complement thereof.
An isolated nucleic acid molecule comprising a nucleotide sequence which has at least 95% identity with the nucleotide sequence of SEQ ID NO:1, or the complement thereof, wherein said nucleic acid molecule encodes only a polypeptide having 6-phosphogluconolactonase activity.
Dependencies are inferred from the claim text.