# US 7,270,812 B2 · Pharmaceutical composition for treatment of cancers

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- Number: US 7,270,812 B2
- Document ID: US7270812B2
- Status: Granted
- Kind: B2
- Application number: 10/911,580
- Filed: 2004-08-05
- Published: 2005-02-10
- Granted: 2007-09-18
- Earliest claimed: 2003-08-08
- Inventors: Yuji Shino; Kouta Sunouchi; Takehide Asano; Hiroshi Shirasawa
- Web page: https://patentgrep.com/patent/US7270812B2

## CPC

- A61K 35/768: Oncolytic viruses not provided for in groups A61K35/761 - A61K35/766
- A61K 35/765: Reovirus; Rotavirus
- A61P 35/00: Antineoplastic agents
- C12N 2720/12032: Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent
- C12N 2770/36132: Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention relates to a pharmaceutical composition for treatment of cancers containing Sindbis virus as an active ingredient, a method for treatment of cancers, which comprises administering a therapeutically effective amount of Sindbis virus to a mammal having cancers, a method for identifying cancers, which includes introducing Sindbis virus into an animal, thereby detecting a Sindbis virus protein or Sindbis virus RNA in vital tissues of the above animal, a method of identifying cancers, which includes introducing into an animal Sindbis virus having a reporter gene incorporated therein, thereby, detecting the product of the reporter gene in vital tissues of the animal, and a method of identifying cancers, which includes detecting a increase in antibody titer to the Sindbis virus protein or the product of the reporter gene in the aforementioned animals.

## Claims

### Claim 1

A pharmaceutical composition for treating pancreatic cancer, which comprises Sindbis virus and reovirus as an active component.

### Claim 2 (depends on 1)

The pharmaceutical composition according to claim 1, which comprises at least one strain of Sindbis virus.

### Claim 3 (depends on 1)

The pharmaceutical composition according to claim 1, wherein the Sindbis virus is Sindbis virus AR339.

### Claim 4

A method for the treatment of pancreatic cancer, which comprises
administering to a mammal having pancreatic cancer a therapeutically effective amount of Sindbis virus and reovirus, whereby tumor size is reduced.

### Claim 5 (depends on 4)

The method according to claim 4, wherein at least one strain of Sindbis virus is administered.

### Claim 6 (depends on 4)

The method according to claim 4, wherein the Sindbis virus is Sindbis virus AR339.

### Claim 7 (depends on 4)

The method according to claim 4, wherein the Sindbis virus is administered intravenously, intraarterially, intramuscularly, intraperitoneally, subcutaneously, topically, intratumorally, orally, transdermally, rectally, intravaginally, intranasally, or sublingually.

### Claim 8 (depends on 4)

The method according to claim 4, wherein 0.01 to 1×1015 plaque forming units (PFU) of Sindbis virus per kilogram of body weight are administered.