Parvovirus vectors and their use
AssigneesDeutsches Krebsforschungszentrum Stiftung des Offentlichen Rechts, Yale University
InventorsJean Rommelaere, Peter Tattersall
Life of this patent
Estimating the term…
Abstract
This invention relates to a parvovirus vector having a parvovirus DNA excisable from the vector DNA in a parvovirus-permissive cell, the parvovirus DNA having a left terminus which comprises a parvovirus minimal origin of replication, and a system comprising the parvovirus vector. Furthermore, this invention concerns a method of producing the parvovirus vector, parvoviral particles as well as their use.
Claims8
A parvovirus vector comprising a parvovirus DNA having a left terminus which comprises a parvovirus minimal origin of replication comprising CTWWTCA, wherein W is any nucleotide, and the parvovirus DNA is excisable from the parvovirus vector in a parvovirus-permissive cell, wherein the parvovirus DNA region coding for capsid proteins is partially or fully replaced by an exogeneous DNA coding for a chemotactic polypeptide.
A system comprising a parvovirus vector and a cell expressing the capsid proteins of parvovirus, wherein the parvovirus vector comprising a parvovirus DNA having a left terminus which comprises a parvovirus minimal origin of replication comprising CTWWTCA, wherein W is any nucleotide, and the parvovirus DNA is excisable from the parvovirus vector in a parvovirus-permissive cell, wherein the parvovirus DNA region coding for capsid proteins is partially or fully replaced by an exogeneous DNA, wherein the expression of the capsid proteins is controlled by a helper plasmid comprising an SV40 origin of replication and the cell expresses an SV40 large T antigen.
Dependencies are inferred from the claim text.