Norovirus infected cell cultures and uses therefor
AssigneeWashington University
InventorsHerbert W. Virgin, Christiane Wobus, Stephanie Karst
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Abstract
A norovirus-permissive cell culture infected with a norovirus, and methods of culturing a norovirus, are disclosed. Norovirus-permissive cells include dendritic cell-lineage cells, and macrophage-lineage cells, such as dendritic cells, and macrophages having a deficiency in a cellular anti-viral pathway such as a STAT-1-dependent pathway, an interferon receptor-dependent pathway, or a PKR-dependent pathway. Also disclosed are methods of screening anti-viral compounds against norovirus-permissive cells infected with norovirus, and norovirus adapted to grow in fibroblasts as well as macrophages that are not deficient in a cellular anti-viral pathway. Methods of making a norovirus vaccine are also disclosed.
Claims14
A norovirus-permissive cell culture infected with a norovirus, the norovirus-permissive cell culture comprising a macrophage infected with a norovirus, wherein the macrophage is deficient in a cellular anti-viral pathway, the anti-viral pathway selected from the group consisting of a STAT-1-dependent anti-viral pathway, an interferon receptor-dependent anti-viral pathway, a double-stranded RNA-dependent serine/threonine protein kinase-dependent anti-viral pathway, and a combination thereof.
A method of replicating a norovirus in vitro, the method comprising:
- inoculating macrophages with the norovirus;
- and
- culturing the macrophages, wherein the macrophages are macrophages deficient in a cellular anti-viral pathway selected from the group consisting of a STAT-1-dependent anti-viral pathway, an interferon receptor-dependent anti-viral pathway, a double-stranded RNA-dependent serine/threonine protein kinase-dependent anti-viral pathway, and a combination thereof.
A norovirus-permissive cell culture infected with a norovirus, wherein the norovirus-permissive cell culture comprises dendritic cells.
A method of replicating a norovirus in vitro, the method comprising:
- inoculating dendritic cells with the norovirus;
- and
- culturing the dendritic cells.
Dependencies are inferred from the claim text.