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US 7,262,054 B2Granted
Zinc finger proteins for DNA binding and gene regulation in plants
AssigneeSangamo BioSciences, Inc.
InventorsAndrew Jamieson, Guofu Li
Life of this patent
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Abstract
Disclosed herein are modified plant zinc finger proteins; compositions comprising modified plant zinc finger proteins and methods of making and using modified plant zinc finger proteins. The modified plant zinc finger proteins, in contrast to naturally-occurring plant zinc finger proteins, have a binding specificity that is determined by tandem arrays of modular zinc finger binding units.
Claims6
A method for modulating gene expression in a plant cell;
- the method comprising contacting the cell with a plant zinc finger protein (ZFP) comprising a tandem array of a plurality of zinc fingers, the plant ZFP having a non-plant ZFP structure and being engineered to bind to a target sequence, wherein the plant ZFP is obtained by assembling sequences from a naturally occurring plant ZFP in a non-plant structure by (i) deleting one or more amino acid residues between the adjacent zinc fingers of the naturally-occurring plant ZFP such that there are between about 5 and 50 amino acids between adjacent zinc fingers of the plant ZFP and (ii) engineering the plant ZFP to bind to the target sequence.
Dependencies are inferred from the claim text.