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US 7,262,046 B2Granted
Aerobic succinate production in bacteria
AssigneeRice University
InventorsSan Ka-Yiu, George N. Bennett, Henry Lin
Life of this patent
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Abstract
Methods of increasing yields of succinate using aerobic culture methods and a multi-mutant E. coli strain are provided. Also provided is a mutant strain of E. coli that produces high amounts of succinic acid.
Claims4
A method of aerobically producing succinate comprising:
- a) generating a genetically engineered E. coli strain comprising:
- i) reduced activity of succinate dehydrogenase (sdhAB), acetate kinase (ackA), phosphotransacetyloase (pta) or both ackA-pta, and
- ii) reduced activity of pyruvate oxidase (poxB);
- and
- iii) reduced activity of aceBAK operon repressor (iclR);
- and
- iv) reduced activity of phosphotransferase system gene (ptsG), and
- v) increased activity of phosphoenolpyruvate carboxylase (pepc);
- and
- b) culturing said bacteria in a fed-batch reactor so that said bacteria produce at least 500 mM succinate.
7 more elements
Dependencies are inferred from the claim text.