Expression of granular starch hydrolyzing enzyme in Trichoderma
AssigneeGenencor International, Inc.
InventorsToby M. Baldwin, Benjamin S. Bower, Nigel Dunn-Coleman, Suzanne E. Lantz, Michael J. Pepsin
Life of this patent
Estimating the term…
Abstract
The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity.
Claims29
A method for producing a granular starch hydrolyzing enzyme having glucoamylase activity (GSHE) in a filamentous fungal host cell comprising
- a) transforming a filamentous fungal host cell with a DNA construct comprising a promoter having transcriptional activity in the filamentous fungal host cell operably linked to a heterologous polynucleotide encoding a GSHE having at least 97% amino acid sequence identity with SEQ ID NO:
- 3,
- b) cultivating the transformed filamentous fungal host cell in a suitable culture medium to allow expression of said GSHE, and
- c) producing the GSHE.
A recombinant Trichoderma cell comprising a heterologous polynucleotide encoding a granular starch hydrolyzing enzyme having glucoamylase activity (GSHE) and at least 97% amino acid sequence identity to SEQ ID NO:
- 3.
A method for producing a granular starch hydrolyzing enzyme having glucoamylase activity (GSHE) in a Trichoderma host cell comprising
- a) transforming a Trichoderma host cell with a DNA construct comprising a promoter having transcriptional activity in the host cell operably linked to a heterologous polynucleotide encoding a GSHE having at least 97% sequence identity to SEQ ID NO:
- 3,
- b) cultivating the transformed Trichoderma host cell to allow expression of said GSHE, and
- c) producing the GSHE.
Dependencies are inferred from the claim text.