Recombinant production of mixtures of antibodies
AssigneeCrucell Holland B.V.
InventorsPatrick Hendrikus Cornelis Van Berkel, Abraham Bout, Ton Logtenberg, Ronald Hendrik Pieter Brus
Life of this patent
Estimating the term…
Abstract
The invention provides methods for producing mixtures of antibodies from a single host cell clone, wherein, a nucleic acid sequence encoding a light chain and nucleic acid sequences encoding different heavy chains are expressed in a recombinant host cell. The recombinantly produced antibodies in the mixtures according to the invention suitably comprise identical light chains paired to different heavy chains capable of pairing to the light chain, thereby forming functional antigen-binding domains. Mixtures of the recombinantly produced antibodies are also provided by the invention. Such mixtures can be used in a variety of fields.
Claims20
A method for producing three or more non-identical antibodies in a single recombinant host cell, the method comprising:
- expressing in said single recombinant host cell one or more nucleic acid sequences encoding a common immunoglobulin light chain and at least three different immunoglobulin heavy chains that are capable of pairing with said common immunoglobulin light chain to form functional antigen binding domains to produce three or more non-identical antibodies that comprise said common light chain.
Dependencies are inferred from the claim text.