Method for modifying plant morphology, biochemistry and physiology
OwnerThomas Schmülling, Tomás Wernerinventors
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Abstract
The present invention provides nucleotide sequences and corresponding amino acid sequences for plant cytokinin oxidase proteins. Also provided are vectors, host cells, and transgenic plants comprising such sequences as well as methods for stimulating root growth and/or enhancing the formation of lateral or adventitious roots and/or altering root geotropism using such sequences. Also provided by the present invention are methods for altering various plant phenotypes including increasing seed size and/or weight, embryo size and/or weight, and cotyledon size and/or weight using cytokinin oxidase proteins and/or nucleic acid molecules encoding cytokinin oxidase.
Claims70
A method for stimulating root growth or for enhancing the formation of lateral or adventitious roots, said method comprising
- introducing into a plant a nucleic acid molecule encoding a plant cytokinin oxidase selected from the group consisting of:
- a an isolated nucleic acid molecule comprising the DNA sequence as set forth in SEQ ID NO:26, or the complement thereof,
- b an isolated nucleic acid molecule comprising the RNA sequence encoding the amino acid sequence of SEQ ID NO:4, or the complement thereof,
- c an isolated nucleic acid molecule encoding the protein comprising the amino acid sequence as set forth in SEQ ID NO:4, or the complement thereof, and
- d an isolated nucleic acid molecule as defined in any of(a) to (c) characterized in that said nucleic acid molecule is DNA, genomic DNA, cDNA, synthetic DNA or RNA wherein T is replaced by U,
- and wherein expression of said nucleic acid molecule stimulates root growth or enhances the formation of lateral or adventitious roots.
An isolated nucleic acid molecule encoding a plant protein having cytokinin oxidase activity selected from the group consisting of:
- a an isolated nucleic acid molecule comprising the contiguous DNA sequence as set forth in SEQ ID NO:
- 26 or the complement thereof,
- b an isolated nucleic acid molecule comprising the RNA sequence encoding the amino acid sequence of SEQ ID NO:4, or the complement thereof,
- c an isolated nucleic acid molecule encoding a protein having the amino acid sequence of SEQ ID NO:32,
- d an isolated nucleic acid molecule encoding the protein having the amino acid sequence of SEQ ID NO:4, or the complement thereof, provided that said nucleic acid molecule is not the nucleic acid molecule as deposited under Genbank accession number:
- AC0059171, whose sequence is set forth in SEQ ID NO:37.
Dependencies are inferred from the claim text.