Genetic engineering salt tolerance in crop plants
OwnerEduardo Blumwald, Maris Apse +2inventors
InventorsEduardo Blumwald, Maris Apse, Wayne Snedden, Gilad Aharon
Life of this patent
Estimating the term…
Abstract
The invention is isolated nucleic acid molecules encoding Na+/H+ exchanger polypeptides with at least 95% homology to that of Arabidopsis thaliana for extrusion of monovalent cations from the cytosol of cells to provide the cell with increased salt tolerance. Crop species transformed with the nucleic acid molecule are capable of surviving in soil with high salt levels that would normally inhibit growth of the crop species.
Claims28
An isolated nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:
- 3 or a nucleic acid that encodes SEQ NO:
- 4.
A tranagenic plant comprising recombinant DNA, wherein the recombinant DNA encodes a polypeptide comprising the peptide sequence of SEQ ID NO:
- 4.
A transgenic plant comprising a recombinant nucleic acid molecule , wherein said nucleic acid molecule comprises
- SEQ ID NO:3 or
- a nucleic acid encoding SEQ ID NO:
- 4.
An expression transgene comprising a recombinant nucleic acid molecule operably linked to a promoter selected from the group consisting of a super promoter, a 35S promoter of cauliflower mosaic virus, a drought-inducible promoter, an ABA-inducible promoter, a heat shock-inducible promoter, a salt-inducible promoter, a copper-inducible promoter, a steroid-inducible promoter and a tissue-specific promoter, wherein said nucleic acid molecule comprises
- SEQ ID NO:
- 3 or
- a nucleic acid encoding SEQ ID NO:
- 4.
A method of producing a geneticaily transformed plant, wherein the method comprises:
- (a) cloning or synthesizing a nucleic acid molecule comprising
- SEQ ID NO:
- 3 or
- a nucleic acid encoding SEQ ID NO:
- 4,
- (b) inserting the nucleic acid molecule into a vector so that the nucleic acid molecule is operably linked to a promoter;
- (c) inserting the vector into a plant cell or plant seed;
- and
- (d) degenerating a plant from the plant cell or plant seed.
Dependencies are inferred from the claim text.