RPSk-1 gene family, nucleotide sequences and uses thereof
AssigneeIowa State University Research Foundation, Inc.
InventorMadan K. Bhattacharyya
Life of this patent
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Abstract
This invention relates to the purified and isolated family of Rps1-k disease resistance genes, proteins encoded thereby and use of the same to confer, enhance or otherwise modify resistance of soybean to plant pathogens, particularly Phytophthora sojae.
Claims17
An isolated nucleic acid molecule that encodes a polypeptide that confers disease resistance activity, said nucleic acid molecule is selected from the group consisting of:
- (a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:
- 11 or SEQ ID NO:
- 13;
- (b) a nucleic acid molecule comprising a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:
- 12 or SEQ ID NO:
- 13;
- and
- c) a nucleic acid molecule comprising a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence set forth in (a).
A method for conferring phytophthora disease resistance in a plant comprising:
- 1) transforming a plant cell with an isolated nucleic acid molecule operably linked to a promoter that regulates transcription of said nucleic acid molecule in a plant cell;
- wherein said nucleic acid molecule encodes a polypeptide that confers phytophthora disease resistance activity and is selected from the group consisting of:
- (a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:
- 11 or SEQ ID NO:
- 13;
- (b) a nucleic acid molecule comprising a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:
- 12 or SEQ ID NO:
- 13;
- and
- b) a nucleic acid molecule comprising a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence set forth in (a);
- and
- 2) regenerating a plant from the transformed plant cell, thereby conferring phytophthora disease resistance in the plant.
A DNA construct comprising a nucleic acid molecule encoding a polypeptide which confers resistance to phytophthora disease;
- said polypeptide is selected from the group consisting of:
- (a) a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 12 or SEQ ID NO:
- 14;
- (b) a polypeptide encoded by the nucleotide sequence of SEQ ID NO:
- 11 or SEQ ID NO:
- 13;
- and
- (c) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the sequence set forth in (a).
Dependencies are inferred from the claim text.