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US 7,256,299 B2Granted
Chemiluminescent reagents
AssigneeEnzo Life Sciences, Inc. C/O Enzo Biochem, Inc.
InventorsJannis G. Stavrianopoulos, Elazar Rabbani
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Abstract
This invention provides for labeling reagents, labeled targets and processes for preparing labeling reagents. The labeling reagents can take the form of cyanine dyes, xanthene dyes, porphyrin dyes, coumarin dyes or composite dyes. These labeling reagents are useful for labeling probes or targets, including nucleic acids and proteins. These reagents can be usefully applied to protein and nucleic acid probe based assays. They are also applicable to real-time detection processes.
Claims16
A chemiluminescent reagent having the structure:
- wherein Q comprises a cycloalkyl or polycycloalkyl group attached covalently to the 4-membered ring portion of said dioxetane above directly or indirectly through a linkage group;
- wherein Z comprises hydrogen, alkyl, aryl, aralkyl, alkaryl, heteroalkyl, heteroaryl, cycloalkyl or cycloheteroalkyl;
- and wherein R1 and R2 comprise chemical moieties attached to different sites of a cyclic ring attached to said dioxetane, and wherein R1 is enzymatically converted into R1* which comprises a chemical reactive group G1, and wherein R2 is attached to said cyclic ring through an oxygen atom and comprises a chemical reactive group G2 which reacts with said G1 to convert said dioxetane to an unstable light-emitting dioxetane form.
A chemiluminescent reagent having the structure:
- wherein Q comprises a cycloalkyl or polycycloalkyl group attached covalently to the 4-membered ring portion of said dioxetane above directly or indirectly through a linkage group;
- wherein Z comprises hydrogen, alkyl, aryl, aralkyl, alkaryl, heteroalkyl, heteroaryl, cycloalkyl or cycloheteroalkyl;
- and wherein R comprises a chemical linker having a reactive site attached to the aromatic ring in said structure;
- and wherein R′ comprises a substrate for an non-cleaving enzymatic process, wherein the product of said enzymatic process leads to further chemical rearrangements that generate an unstable light emitting dioxetane form.
1 more element
Dependencies are inferred from the claim text.