Sialyltransferase and DNA encoding the same
AssigneeSeikagaku Kogyo Kabushiki Kaisha
InventorMasaki Saito
Life of this patent
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Abstract
A sialyltransferase having the following physico-chemical properties; (1) Activity: transfers sialic acid from a sialic acid donor selectively to a 3-hydroxyl group of a galactose residue contained in lactosylceramide as a sialic acid acceptor to produce ganglioside GM3; (2) Optimal reaction pH: 6.0 to 7.0; and (3) Inhibition and activation: the activity increases at least 1.5 times with 10 mm of Mn2+ as compared with the case in the absence thereof.
Claims4
An isolated DNA encoding all or a sialyltransferase-active part of a sialyltransferase having an amino acid sequence shown by SEQ ID NO:
- 2.
An isolated DNA which has a nucleotide sequence of base numbers 202 to 1278 in a nucleotide sequence shown by SEQ ID NO:
- 1.
An isolated DNA which hybridizes with a polynucleotide having a nucleotide sequence complementary to a nucleotide sequence shown by SEQ ID NO:
- 1 when hybridization is performed in a solution containing 50% formamide, 5×SSPE, 5×Denhart's solution, 0.5% SDS and 50 μg/ml of denatured salmon sperm DNA at 42° C. for 16 hours, and then washing is performed sequentially with 1×SSPE, 1% SDS and with 0.1×SSPE, 0.1% SDS at 55° C., said DNA encoding a sialyltransferase.
An isolated DNA which hybridizes with a polynucleotide having a nucleotide sequence complementary to a nucleotide sequence of base numbers 202 to 1278 in the nucleotide sequence shown by SEQ ID NO:
- 1 when hybridization is performed in a solution containing 50% formamide, 5×SSPE, 5×Denhart's solution, 0.5% SDS and 50 μg/ml of denatured salmon sperm DNA at 42° C. for 16 hours, and then washing is performed sequentially with 1×SSPE, 1% SDS and with 0.1×SSPE, 0.1% SDS at 55° C., said DNA encoding a sialyltransferase.
Dependencies are inferred from the claim text.