Devices and methods for separating phospholipids from biological samples
AssigneeTandem Labs.
InventorsPatrick Kevin Bennett, Kenneth Charles Van Horne
Life of this patent
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Abstract
Device and methods for the removal of phospholipids from biological samples are disclosed and described. Removal of phospholipids may be desirable for the analysis of the phospholipids themselves, or to prevent the phospholipids from conflicting with and effectively masking other analytes in the sample for which identification or quantification is sought.
Claims49
A device for removing phospholipids from a biological sample, comprising:
- a) a support;
- and
- b) at least one type of phospholipotropic multivalent cation coupled to the support in a concentration that is sufficient to capture and retain phospholipids from the biological sample said cation including either an actinide, or a lanthanide or a mixture thereof, said lanthanide being selected from the group consisting of:
- cerium, lanthanum, europium, neodymium, gadolinium, or a mixture thereof.
A method of removing phospholipids from a biological sample, comprising:
- a) contacting at least one type of phospholipotropic multivalent cation selected from the group consisting of lanthanides and actinides, and coupled to a support, with the biological sample;
- b) capturing phospholipids in the sample with the cation by chemically coordinating including chelating or ionically associating the phospholipids with the cation;
- and
- c) separating the cation and captured phospholipids from the sample.
A method of making a device for removing phospholipids from a biological sample, comprising:
- coupling at least one type of phospholipotropic multivalent cation with a support by a mechanism selected from the group consisting of ionic bonding, covalent bonding, chemical coordination including chelation, and combinations thereof, in a manner that preserves an affinity of the cation for phospholipids and in concentration that is sufficient to capture and retain phospholipids from the biological sample, said cation including either an actinide, or a lanthanide or a mixture thereof, said lanthanide being selected from the group consisting of:
- cerium, lanthanum, europium, neodymium, gadolinium, or a mixture thereof.
A device for removing phospholipids from a biological sample, comprising:
- a) an inorganic salt matrix support;
- and
- b) at least one type of phospholipotropic multivalent cation selected from the group consisting of lanthanides and actinides coupled to the inorganic salt matrix support by a mechanism selected from the group consisting of ionic bonding, covalent bonding, chemical coordination including chelation, and combinations thereof,
- in a concentration that is sufficient to capture and retain phospholipids from the biological sample.
A method of removing phospholipids from a biological sample, comprising:
- a) contacting at least one phospholipotropic multivalent cation selected from the group consisting of lanthanides or actinides coupled to a support with the biological sample;
- b) capturing phospholipids in the sample with the cation;
- and
- c) separating the cation and captured phospholipids from the sample.
Dependencies are inferred from the claim text.