Isolation, selection and propagation of animal transgenic stem cells
AssigneeThe University of Edinburgh
InventorsAustin Gerard Smith, Peter Scott Mountford
Life of this patent
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Abstract
Animal stem cells are obtained and maintained by culturing cells containing, in the genome, a selectable marker. Differential expression of the selectable marker enables preferential survival and/or division of the desired stem cells compared to the non-stem cells.
Claims25
A method of isolating mammalian stem cells from a population of mammalian cells, comprising:
- (a) culturing mammalian cells containing a nucleic acid construct including a selectable marker operably linked to a promoter that preferentially expresses the selectable marker in mammalian stem cells;
- and
- (b) isolating mammalian stem cells expressing the selectable marker.
An isolated mammalian stem cell comprising at least one nucleic acid construct encoding a selectable marker operatively linked to a promoter which preferentially expresses said marker in said mammalian stem cell.
An isolated mammalian stem cell comprising at least one nucleic acid construct encoding a fluorescent marker operatively linked to a promoter which preferentially expresses said marker in said mammalian stem cell.
A vector comprising a nucleic acid sequence encoding a selectable marker operatively linked to a promoter that preferentially expresses the selectable marker in mammalian stem cells.
Dependencies are inferred from the claim text.