Polypeptide display libraries and methods of making and using thereof
AssigneeThe Regents of the University of California
InventorsPatrick S. Daugherty, Paul H. Bessette, Jeffrey Rice
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Abstract
Disclosed herein are expression vectors which display a passenger polypeptide on the outer surface of a biological entity. As disclosed herein the displayed passenger polypeptide is capable of interacting or binding with a given ligand. Also disclosed are methods of making and using the expression vectors. N/C terminal fusion expression vectors and methods of making and using are also disclosed.
Claims21
An expression vector capable of genetically expressing and displaying a given passenger polypeptide in a carrier protein that is an extracellular loop of a bacterial outer membrane protein to result in (a) the passenger polypeptide being capable of interacting with a given ligand, and (b) a terminus of the extracellular loop, on an outer surface of a biological entity.
Dependencies are inferred from the claim text.