US 7,256,031 B2 · Enzyme having β-glucosidase activity and use thereof

Abstract

The invention is to provide a novel β-glucosidase and a gene that codes for the enzyme, and to develop a technique of utilizing the β-glucosidase or a composition that contains the enzyme for processing plants or plant-derived substances. According to the invention, there are provided a novel enzyme showing a β-glucosidase activity and derived from filamentous fungi of the genus Acremonium, a gene that codes for the enzyme, a method of using the gene for expressing β-glucosidase, an enzyme composition that contains β-glucosidase, and a method of processing plants or plant-derived substances with the enzyme or the enzyme compositions.

Claims65

Dependent claims

An enzyme having β-glucosidase activity, which is isolated from Acremonium cellulolyticus and has the following characteristics:

(a) substrate specificity, action characteristics:
acts on cello-oligosaccharides and on glycosides with aglycone linked thereto via β-D-glucopyranosyl linkage to produce glucose;
(b) molecular weight:
108,000 by SDS-polyacrylamide gel electrophoresis;
(c) isoelectric point:
pI 4.7 by polyacrylamide gel isoelectric focusing;
(d) optimum pH:
between pH 3.5 and 4.0;
(e) optimum temperature for action:
70° C.;
and
(f) comprises amino acids 1 to 838 of the amino acid sequence of SEQ ID NO:
2 of the Sequence Listing.

10 more elements

Dependencies are inferred from the claim text.

Enzyme having β-glucosidase activity and use thereof

Life of this patent

Enzyme having β-glucosidase activity and use thereof

Life of this patent

Abstract

Claims65