Gene improving temperature-tolerance of acetic acid bacterium, acetic acid bacterium bred using the gene and process for producing vinegar using the acetic acid bacterium
AssigneeMitsukan Group Corporation
InventorHidetsugu Goto
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Abstract
The present invention is intended to provide a novel gene originating in an acetic acid bacterium, which participates in temperature tolerance; a method of improving the temperature tolerance of a microorganism, in particular, an acetic acid bacterium using the above gene; and a method of efficiently producing vinegar having a higher acetic acid concentration with the use of an acetic acid bacterium whose temperature tolerance has been improved.
Claims17
An isolated polynucleotide which encodes a polypeptide comprising SEQ ID NO:
- 2 that enhances temperature tolerance of Acetobacter or Gluconacetobacter;
- wherein growth and/or acetic acid fermentation of an Acetobacter or Gluconacetobacter transformed with said polynucleotide is enhanced at a temperature above the optimal fermentation temperature for the corresponding untransformed strain.
The isolated polynucleotide consisting of nucleotides 73 to 1251 of SEQ ID NO:
- 1.
The recombinant plasmid pUCGCS deposited as FERM BP-8217.
An isolated polynucleotide which encodes a polypeptide comprising SEQ ID NO:
- 2 or a fragment of SEQ ID NO:
- 2 that enhances temperature tolerance when expressed in Acetobacter or Gluconacetobacter;
- wherein growth and/or acetic acid fermentation of an Acetobacter or Gluconacetobacter transformed with said polynucleotide is enhanced at a temperature above the optimal fermentation temperature for the corresponding untransformed strain.
Dependencies are inferred from the claim text.