Terminal phosphate blocked nucleoside polyphosphates
AssigneeGE Healthcare Bio-Sciences AB
InventorsAnup Sood, Shiv Kumar, Carl Fuller, John Nelson
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Abstract
The present invention describes terminal phosphate blocked nucleoside polyphosphates that are stable at high temperature and their use in nucleic acid amplification and analysis. Current invention further describes charge modified terminal phosphate blocked nucleoside polyphosphates for improved incorporation and direct loading of nucleic acid sequencing reactions onto separating media.
Claims14
In a method for nucleic acid analysis, which includes a high temperature polymerase reaction of a nucleic acid template, a primer, a nucleic acid polymerase, and at least one nucleoside polyphosphate, the improvement comprising conducting said polymerase reaction in the presence of at least one thermally stable terminally blocked nucleoside polyphosphates, wherein said nucleic acid analysis is chain terminator DNA sequencing.
Dependencies are inferred from the claim text.