Kit for determining the acetylation level of a peptide based on sensitivity of the peptide to peptidase
AssigneeCyclex, Inc.
InventorsKatsuyuki Tamai, Toshiaki Miyazaki, Emi Wada, Ayumi Tatsuzawa
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Abstract
The acetylation level of a peptide is determined utilizing the fact that the changes in the acetylation level are reflected in the sensitivity of the substrate peptide to a peptidase. This method can be used for measuring activities of deacetylase and acetylase, and also enables screening for substances that influence the activity of these enzymes. The deacetylase activity can be measured by a simple procedure according to the present invention.
Claims14
A kit for use in determining a deacetylation activity of a sample containing a deacetylase, which comprises:
- (a) an acetylated substrate peptide labeled with a substance which emits a signal;
- and
- (b) a peptidase whose peptide-cleaving activity changes upon an acetylation level of the substrate peptide.
A kit for use in identifying a compound that inhibits or enhances a deacetylation activity of a deacetylase, which comprises:
- (c) an acetylated substrate peptide labeled with a substance which emits a signal;
- (d) a deacetylase;
- and
- (e) a peptidase whose peptide-cleaving activity changes upon an acetylation level of the substrate peptide.
A kit for use in determining an acetylation activity of a sample containing an acetylase, which comprises:
- (a) a substrate peptide labeled with a substance which emits a signal;
- and
- (b) a peptidase whose peptide-cleaving activity changes upon an acetylation level of the substrate peptide.
Dependencies are inferred from the claim text.