Methods of regulating cytokine signalling

A method for identifying a modulator of interaction between a human or murine SOCS-3 molecule and a gp130 receptor, said method comprising:

• immobilizing said gp130 receptor or a part thereof to a solid support, wherein said part comprises the cytoplasmic domain of said gp130 receptor or at least SEQ ID NO:5;
• contacting the immobilized gp130 receptor or said part thereof with said SOCS-3 molecule or a part of said SOCS-3 in the presence of a potential agonist or antagonist, wherein said part of said SOCS-3 comprises the SH2 domain of said SOCS-3;
• and
• measuring qualitatively or quantitatively a change in binding between said SOCS-3 or said part of said SOCS-3 and said gp130 receptor or said part of said gp130 receptor, as compared to binding in the absence of said potential agonist or antagonist, thereby identifying a modulator of interaction between said SOCS-3 molecule and said gp130 receptor.

A method for identifying a modulator of interaction between a human or murine SOCS-3 molecule and a gp130 receptor, said method comprising:

• immobilizing said SOCS-3 molecule or a part thereof to a solid support via binding partners comprising biotin and streptavidin, wherein said part comprises the SH2 domain of said SOCS-3 molecule, and wherein one of said biotin or streptavidin is attached to said solid support and the other is linked to said SOCS-3 molecule or said part;
• contacting the immobilized SOCS-3 molecule or said part with said gp130 receptor or a part of said gp130 receptor in the presence of a potential agonist or antagonist, wherein said part comprises the cytoplasniic domain of said gp130 receptor or at least SEQ ID NO:5;
• and
• measuring qualitatively or quantitatively a change in binding between said SOCS-3 or said part of said SOCS-3 and said gp130 receptor or said part of said gp130 receptor, as compared to binding in the absence of said potential agonist or antagonist, thereby identifying a modulator of interaction between said SOCS-3 molecule and said gp130 receptor.

A method of identifying a modulator of interaction between a human or murine SOCS-3 molecule and a gp130 receptor, said method comprising:

• immobilizing said gp130 receptor or a part thereof to a solid support via binding partners comprising biotin and streptavidin, wherein said part comprises the cytoplasmic domain of said gp130 receptor or at least SEQ ID NO:5, and wherein one of said biotin or streptavidin is attached to said solid support and the other of said biotin or streptavidin is linked to said gp130 receptor or said part thereof;
• contacting the immobilized gp130 receptor or said part thereof with said SOCS-3 molecule or a part of said SOCS-3 molecule in the presence of a potential agonist or antagonist, wherein said part of said SOCS-3 molecule comprises the SH2 domain of said SOCS-3 molecule;
• and
• measuring qualitatively or quantitatively a change in binding between said SOCS-3 or said part of said SOCS-3 and said gp130 receptor or said part of said gp130 receptor, as compared to binding in the absence of said potential agonist or antagonist, thereby identifying a modulator of interaction between said SOCS-3 molecule and said gp130 receptor.