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US 7,255,996 B2Granted
Compositions, methods and kits for detecting the nucleic acids of HIV-1 and HIV-2
AssigneeGen-Probe Incorporated
InventorsJeffrey M. Linnen, Wen Wu
Life of this patent
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Abstract
Compositions, methods and kits for detecting the nucleic acids of HIV-1, HIV-2, or the combination of HIV-1 and HIV-2. Particularly described are oligonucleotides that are useful as hybridization probes and amplification primers, including cross-reacting hybridization probes and cross-reacting amplification primers, for detecting very low levels of viral nucleic acids.
Claims13
A method of determining whether a test sample contains at least one of HIV-1 nucleic acid and HIV-2 nucleic acid, said method comprising the steps of:
- (a) combining said test sample with a pair of cross-reactive primers to form a reaction mixture, wherein said pair of cross-reactive primers is capable of co-amplifying HIV-1 and HIV-2 nucleic acid and comprises a first primer sequence that consists of SEQ ID NO:14, optionally comprising an upstream sequence that is not complementary to either HIV-1 or HIV-2 nucleic acid, and a second primer sequence that consists of SEQ ID NO:7, optionally comprising an upstream sequence that is not complementary to either HIV-1 or HIV-2 nucleic acid;
- (b) amplifying, in an in vitro nucleic acid amplification reaction, nucleic acid present in said reaction mixture of step (a);
- and
- (c) detecting in a hybridization reaction an HIV-1 amplicon, an HIV-2 amplicon, or a combination of both amplicons synthesized in step (b), thereby determining that said test sample contains at least one of HIV-1 nucleic acid and HIV-2 nucleic acid.
1 more element
Dependencies are inferred from the claim text.