Method for obtaining nucleic acids from an environment sample, resulting nucleic acids and use in synthesis of novel compounds

The method for collecting nucleic acids from organisms in a soil sample comprising:

• (a) grinding a pre-dried or pre-desiccated soil sample containing organisms to produce microparticles;
• (b) suspending the microparticles in a liquid buffer medium;
• (c) extracting nucleic acids from the organisms in the suspension of microparticles;
• (d) passing the extracted nucleic acids over a molecular sieve to produce elution fractions enriched in nucleic acids;
• (e) passing the elution fractions enriched in nucleic acids over an anion-exchange chromatography support to produce fractions containing substantially purified nucleic acids;
• and
• recovering the elution fractions containing the substantially purified nucleic acids;
• wherein:
• step (b) further comprises:
• homogenizing the microparticles by vigorous mixing followed by simple stirring;
• incubating the suspension at 37° C., after sonication, in the presence of lysozyme and achromopeptidase;
• and
• adding SDS to the suspension;
• wherein:
• the one or more purified nucleic acids in the vector comprise SEQ ID No.:
• 34;
• and
• the host cell is cultured under conditions suitable to produce a polypeptide encoded by the nucleic acid in the vector;
• and
• wherein the vector is inserted into a host cell.