Continuous direct enzymatic protein solubilization process for industrial wastes

A process for treating an industrial waste of plant origin, the industrial waste comprising an insoluble starting protein, the process consisting of the following steps:

• (a) pretreating the waste with successive washings of acidified water, at a pH range of 4 to 5.5, in order to wash out alkaloids,
• (b) enzymatically reacting said waste with an endoprotease to attain a degree of hydrolysis ranging from 25 to 40% to obtain a liquid paste comprising a first product that is a liquid phase consisting essentially of solubilized peptones at a concentration ranging from 60 to 80% of the insoluble starting protein, and a second product that is a solid phase consisting essentially of non-solubilized proteins, starches, and insoluble sugars, wherein the enzymatic reacting step follows in a continuous process,
• (c) clarifying the liquid paste separately to recover the first and second products,
• (d) evaporating the liquid phase until a liquid syrup containing from 40 to 70% solid content is formed and, optionally,
• (e) subjecting the liquid syrup to a cleavage of the syrup into a liquid phase substance with a majority protein content and a solid phase substance with a majority lipid content.

A process for treating an industrial waste, the industrial waste comprising an insoluble starting protein, the process consisting of the steps of:

• (a) enzymatically reacting said waste with an endoprotease to attain a degree of hydrolysis ranging from 25 to 40% to obtain a liquid paste comprising a first product that is a liquid phase consisting essentially of solubilized peptones at a concentration ranging from 60 to 80% of the insoluble starting protein, and a second product that is a solid phase consisting essentially of non-solubilized proteins, starches, and insoluble sugars
• (b) clarifying the liquid paste to separately recover the first and second products;
• (c) recovering the second product and
• (d) feeding it to an animal.

A process for treating an industrial waste, the industrial waste comprising an insoluble starting protein, the process consisting of the steps of:

• (a) enzymatically reacting said waste with an endoprotease to attain a degree of hydrolysis ranging from 25 to 40% to obtain a liquid paste comprising a first product that is a liquid phase consisting essentially of solubilized peptones at a concentration ranging from 60 to 80% of the insoluble starting protein, and a second product that is a solid phase consisting essentially of non-solubilized proteins, starches, and insoluble sugars
• (b) clarifying the liquid paste to separately recover the first and second products;
• (c) recovering the solubilized peptones and
• (d) introducing them as a nitrogen source into a culture media for fermentation.

A process for treating an industrial waste, the industrial waste comprising an insoluble starting protein, the process consisting of the steps of:

• (a) enzymatically reacting said waste with an endoprotease to attain a degree of hydrolysis ranging from 25 to 40% to obtain a liquid paste comprising a first product that is a liquid phase consisting essentially of solubilized peptones at a concentration ranging from 60 to 80% of the insoluble starting protein, and a second product that is a solid phase consisting essentially of non-solubilized proteins, starches, and insoluble sugars
• (b) clarifying the liquid paste to separately recover the first and second products;
• (c) recovering the solubilized peptones and
• (d) introducing them as an additive into a food composition.

A process for treating an industrial waste, the industrial waste comprising an insoluble starting protein, the process consisting of the steps of:

• (a) enzymatically reacting said waste with an endoprotease to attain a degree of hydrolysis ranging from 25 to 40% to obtain a liquid paste comprising a first product that is a liquid phase consisting essentially of solubilized neptones at a concentration ranging from 60 to 80% of the insoluble starting protein, and a second product that is a solid phase consisting essentially of non-solubilized proteins, starches, and insoluble sugars
• (b) clarifying the liquid paste to separately recover the first and second products;
• (c) recovering the solubilized peptones and
• (b) introducing them as an additive into a cosmetic composition.

A process for treating an industrial waste, the industrial waste comprising an insoluble starting protein, the process consisting of the steps of:

• (a) enzymatically reacting said waste with an endoprotease to attain a degree of hydrolysis ranging from 25 to 40% to obtain a liquid paste comprising a first product that is a liquid phase consisting essentially of solubilized peptones at a concentration ranging from 60 to 80% of the insoluble starting protein, and a second product that is a solid phase consisting essentially of non-solubilized proteins, starches, and insoluble sugars
• (b) clarifying the liquid paste to separately recover the first and second products;
• (c) recovering the solubilized peptones and
• (d) introducing them as an ingredient in organic fertilizer.

A process for treating an industrial waste of plant origin, the industrial waste comprising an insoluble starting protein, the process consisting of the following steps:

• (a) pretreating the waste with successive washings of acidified water, at a pH range of 4 to 5.5, in order to wash out alkaloids,
• (b) enzymatically reacting said waste with an endoprotease to attain a degree of hydrolysis ranging from 25 to 40% to obtain a liquid paste comprising a first product that is a liquid phase consisting essentially of solubilized peptones at a concentration ranging from 60 to 80% of the insoluble starting protein, and a second product that is a solid phase consisting essentially of non-solubilized proteins, starches, and insoluble sugars, wherein the enzymatic reacting step follows in a continuous process,
• (c) clarifying the liquid paste separately to recover the first and second products,
• (d) evaporating the liquid phase until a liquid syrup containing from 40 to 70% solid content is formed and, optionally,
• (e) subjecting the liquid syrup to a cleavage of the syrup into a liquid phase substance with a majority protein content and a solid phase substance with a majority lipid content;
• and
• (f) combining the liquid phase product and the solid phase product to form an antioxidant.

A process for treating an industrial waste of plant origin, the industrial waste comprising an insoluble starting protein, the process consisting of the following steps:

• (a) pretreating the waste with successive washings of acidified water, at a pH range of 4 to 5.5, in order to wash out alkaloids,
• (b) enzymatically reacting said waste with an endoprotease to attain a degree of hydrolysis ranging from 25 to 40% to obtain a liquid paste comprising a first product that is a liquid phase consisting essentially of solubilized peptones at a concentration ranging from 60 to 80% of the insoluble starting protein, and a second product that is a solid phase consisting essentially of non-solubilized proteins, starches, and insoluble sugars, wherein the enzymatic reacting step follows in a continuous process,
• (c) clarifying the liquid paste separately to recover the first and second products,
• (d) evaporating the liquid phase until a liquid syrup containing from 40 to 70% solid content is formed and, optionally,
• (e) subjecting the liquid syrup to a cleavage of the syrup into a liquid phase substance with a majority protein content and a solid phase substance with a majority lipid content;
• and
• (f) introducing the liquid phase product and the solid phase product into a cosmetic or pharmaceutical cream as an ingredient.