Scanning microscope system
AssigneeOlympus Corporation
InventorShinji Motomura
Life of this patent
Estimating the term…
Abstract
A scanning microscope system includes an optical microscope observation unit that irradiates the sample with excitation light, and forms an optical image from fluorescence emitted from a sample. The system also includes a scanning map creator and a flying spot scanning observation unit. The scanning map creator creates a scanning map indicating a scanning region in which a substance to be scanned exists in the sample, based on brightness of pixels of the optical image. The flying spot scanning observation unit scans the scanning region of the sample with laser beam, and forms a scanning image based on fluorescence emitted from the sample.
Claims14
A scanning microscope system comprising:
- an optical microscope observation unit that irradiates a sample with excitation light, and forms an optical image from fluorescence emitted from the sample;
- a scanning map creator that creates a scanning map indicating a scanning region in which a substance to be scanned exists in the sample, based on a brightness of pixels of the optical image formed by the optical microscope observation unit;
- and
- a flying spot scanning observation unit that scans only the scanning region of the sample with a laser beam, and forms a scanning image based on fluorescence emitted from the sample, wherein the scanning region is determined based on the scanning map created by the scanning map creator.
A scanning microscope system comprising:
- an optical microscope observation unit that irradiates a sample with excitation light, and forms an optical image from fluorescence emitted from the sample;
- a scanning map creator that creates a scanning map indicating a scanning region in which a substance to be scanned exists in the sample, based on a brightness of pixels of the optical image formed by the optical microscope observation unit;
- and
- a flying spot scanning observation unit that scans the scanning region of the sample with a laser beam, and forms a scanning image based on fluorescence emitted from the sample, wherein the scanning region has a number of pixels with brightness higher than a threshold, the number being larger than a predetermined number.
A scanning microscope system comprising:
- an optical microscope observation unit that irradiates a sample with excitation light, and forms an optical image from fluorescence emitted from the sample;
- a scanning map creator that creates a scanning map indicating a scanning region in which a substance to be scanned exists in the sample, based on a brightness of pixels of the optical image formed by the optical microscope observation unit;
- and
- a flying spot scanning observation unit that scans the scanning region of the sample with a laser beam, and forms a scanning image based on fluorescence emitted from the sample;
- wherein the optical microscope observation unit includes a spinning disk inserted into a confocal position on an optical path for observation of the sample, and acquires the optical image by a confocal method, and wherein the spinning disk includes an optical transmission portion.
Dependencies are inferred from the claim text.