Drosophila strain carrying bradeion gene(s) transferred thereinto
AssigneeNational Institute of Advanced Industrial Science and Technology
InventorsManami Tanaka, Masamitsu Yamaguchi
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Abstract
Provided is a special Drosophila strain having a Bradeion gene artificially incorporated therein to exclusively express the gene in the compound eyes and thus to exhibit rough eye. The Drosophila strain has the following properties: (a) a human-derived Bradeion gene has been transferred in the strain to express the human-derived protein Bradeion in the compound eyes from the developmental period; (b) the compound eyes show morphological abnormality due to the expression of Bradeion; and (c) the morphological abnormality occurring in the compound eyes is multiplied according to the number of transferred Bradeion genes.
Claims1
A transgenic Drosophila melanogaster strain whose genome comprises (i) a first transgene, comprising a cDNA encoding a human β-Bradeion protein operably linked to a promoter comprising a UAS, and (ii) a second transgene, comprising a cDNA encoding a GAL4 protein operably linked to a Glass promoter, wherein a GAL4 binding site in the promoter is bound by the Gal4 protein, expressed specifically in compound eyes, and wherein
- (a) said Bradeion protein is expressed in the compound eyes during development thereof;
- and
- (b) the compound eyes show rough eye, in which the cell cycles of each single eye are inconsistent due to the expression of said Bradeion protein.
Dependencies are inferred from the claim text.