Muteins of human neutrophil gelatinase-associated lipocalin and related proteins
AssigneePieris AG
InventorsArne Skerra, Steffen Schlehuber
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Abstract
Disclosed is a method for generating a mutein of a protein selected from the group consisting of human neutrophil gelatinase-associated lipocalin (hNGAL), rat α2-microglobulin-related protein (A2m) and mouse 24p3/uterocalin (24p3), said mutein having detectable affinity to a given target. The method comprises the step of subjecting the protein to mutagenesis at one or more of the sequence positions which correspond to the sequence positions 33 to 54, 66 to 83, 94 to 106, and 123 to 136 of hNGAL, resulting in one or more mutein(s) of the protein. Also disclosed are muteins obtainable by this method.
Claims16
A method for generating a mutein of a protein selected from the group consisting of human neutrophil gelatinase-associated lipocalin (hNGAL), rat α2-microglobulin-related protein (A2m) and mouse 24p3/uterocalin (24p3), said mutein having detectable affinity to a given target, comprising:
- subjecting the protein to mutagenesis at one or more of the sequence positions which correspond to the sequence positions 40 to 50, 70 to 79, 101 to 103, and 125 to 132 of hNGAL, resulting in one or more mutein(s) of the protein.
A mutein derived from a protein selected from the group consisting of:
- human neutrophil gelatinase-associated lipocalin (hNGAL), rat α2-microglobulin-related protein (A2m) and mouse 24p3/uterocalin (24p3), wherein the mutein comprises a mutation at one or more of the sequence positions corresponding to sequence positions 40 to 50, 70 to 79, 101 to 103, and 125 to 132 of hNGAL, and wherein the mutein has detectable binding affinity for a given target.
Dependencies are inferred from the claim text.