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US 7,252,995 B2Granted
Method of generating neurons from stem cells and medium for culturing stem cells
OwnerYu-Show Fu, Henrich Chenginventors
Life of this patent
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Abstract
The present invention relates to a method of generating neurons from stem cells which comprises culturing neurons in a medium and culturing the stem cells in the resultant mixture. The present invention also relates to a medium for culturing stem cells prepared by culturing neurons in a base culture medium.
Claims12
A method of generating neurons from stem cells which comprises:
- (1) culturing neurons obtained from hippocampus in a culture medium, and (2) subsequently culturing stem cells in the medium taken from the culture of the neurons, wherein the stem cells are selected from the group consisting of embryonic stem (ES) cells, embryonic germ (EG) cells, embryonic carcinoma (EC) cells, bone marrow stromal cells (BMSC), bone marrow hematopoietic cells, umbilical cord blood cells and umbilical mesenchymal stem cells.
A medium for culturing stem cells, which is prepared by the process of (a) culturing neurons obtained from hippocampus in a base culture medium for 3-6 days, (b) removing the neurons from the medium, and (c) storing the medium for future use for culturing stem cells.
Dependencies are inferred from the claim text.