Adenovirus supervector system
AssigneeBoard of Regents, The University of Texas System
InventorsWei-Wei Zhang, Jack Roth
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Abstract
An adenoviral supervector system is disclosed that is capable of expressing more than 7.5 kilobases of heterologous DNA in a replication defective adenoviral vector. The supervector system comprises an adenoviral vector construct and a helper cell. The vector construct is capable of being replicated and packaged into a virion particle in the helper cell. In particular, the helper cell expresses DNA from the E2 region of the adenovirus 5 genome and complements deletions in that region in the vector construct. In certain embodiments, the disclosed invention comprises tissue specific expression of up to 30 kb of heterologous DNA directed by an adenoviral vector. Also disclosed are methods of transferring heterologous DNA into mammalian cells.
Claims22
An adenovirus vector construct, wherein all of the E2 coding region has been deleted from the adenovirus genome and heterologous DNA is inserted in its place, but excluding an adenovirus vector from which each of the E1, E2, E3 and E4 coding regions have been deleted.
An adenovirus vector construct consisting essentially of map units 0-1.25 of the adenovirus 5 genome, at least 7.5 kb of heterologous DNA and map units 84.5-100 of the adenovirus 5 genome.
Dependencies are inferred from the claim text.