Attenuated mutant newcastle disease virus strains for in ovo vaccination, method for preparing and their use
AssigneeCentrum Voor Onderzoek in Diergeneeskunde en Agrochemie
InventorsJan Mast, Guy Meulemans
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Abstract
The present invention relates to new attenuated mutant New Castle's disease La SotaNewcastle disease virus strains suitable for in ovo vaccination of avian species comprising a mutation in the gene sequences encoding the HN and/or F glycoproteins of said virus. Furthermore, the invention relates to a vaccine composition comprising said attenuated mutant Newcastle's disease La Sota virus strain, and to the use thereof for the preparation of a vaccine for in ovo vaccination of avian species against Newcastle's disease.
Claims8
An isolated attenuated mutant Newcastle disease La Sota virus suitable for in ovo vaccination of avian species comprising a mutation in the gene sequences encoding the HN and/or F glycoproteins of the virus, wherein the virus comprises a mutation selected from
- an Asp72Glu substitution in the F glycoprotein;
- a Leu160Gln, Leu193Ser or Leu229Arg substitution in the HN glycoprotein;
- and an Asp72Tyr substitution in the F glycoprotein and a Leu229Arg substitution in the HN glycoprotein.
An isolated attenuated mutant Newcastle disease La Sota virus strain chosen from the strains as deposited as La Sota mutant 1C3+8C11, under registration number CNCM I-2714, in the National Collection of Cultures of Microorganisms of the Pasteur institute in Paris.
Dependencies are inferred from the claim text.