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US 7,252,983 B2Granted
Microbially-expressed thermotolerant phytase for animal feed
AssigneeSyngenta Participations AG
InventorsMichael B. Lanahan, Edward Koepf, Keith Kretz
Life of this patent
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Abstract
The invention provides methods for making and using thermotolerant phytases, e.g., a method of using a thermotolerant phytase in feed and food processing and feed or food products comprising a thermotolerant phytase.
Claims11
A method of preparing animal feed, comprising:
- a) providing a mixture comprising a feed component and a preparation comprising a thermotolerant phytase which retains at least 40% activity after 30 minutes at 60° C. and has a specific activity of greater than 200 U/mg at pH 4.5 and 37° C., wherein the thermotolerant phytase is encoded by a nucleic acid molecule comprising the nucleic acid sequence depicted in SEQ ID NO:4 encompassed by the restriction enzyme sites XhoI and NotI or a conservative variant thereof,
- or wherein the thermotolerant phytase comprises the polypeptide sequence of SEQ ID NO:1 or a conservative variant thereof;
- and b) treating the mixture under conditions of temperature and moisture which hydrolyze phytic acid present in the mixture so as to yield animal feed.
A method of preparing animal feed, comprising:
- a) providing a mixture comprising animal feed components and a thermotolerant phytase which retains at least 40% activity after 30 minutes at 60° C. and has a specific activity of greater than 200 U/mg at pH 4.5 and 37° C., wherein the thermotolerant phytase is encoded by a nucleic acid molecule comprising the nucleic acid sequence depicted in SEQ ID NO:4 encompassed by the restriction enzyme sites XhoI and NotI or a conservative variant thereof,
- or wherein the thermotolerant phytase comprises the polypeptide sequence of SEQ ID NO:1 or a conservative variant thereof;
- and b) heating the mixture at a temperature greater than 50° C. so as to yield a heat-treated animal feed mixture.
Dependencies are inferred from the claim text.