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AssigneeMartek Bioscience Corporation
InventorsPaul W. Behrens, John M. Thompson, Kirk Apt, Joseph W. Pfeifer, III, James P. Wynn, James Casey Lippmeier, Jaouad Fichtali, Jon Hansen
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Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
A method of producing docosahexaenoic acid (DHA) by culturing heterotrophic microalgae of the class Dinophyceae in a culture medium, wherein the culture medium has a pH of less than about 6.0, wherein the microalgae produce at least about 0.04 g DHA per liter of 7-day culture, and wherein the medium further comprises:
Dependencies are inferred from the claim text.