Method for producing L-arginine
AssigneeAjinomoto Co., Inc.
InventorsMikiko Yamaguchi, Hisao Ito, Yoshiya Gunji, Hisashi Yasueda
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Abstract
L-Arginine is produced by culturing a microorganism which has L-arginine producing ability and has been modified so that expression of lysE gene should be enhanced, such a microorganism further modified so that an arginine repressor should not function normally, or such a microorganism further modified so that intracellular activity of an enzyme in L-arginine biosynthetic pathway should be enhanced in a medium to produce and accumulate L-arginine in the medium and collecting the L-arginine from the medium.
Claims5
A method for producing L-arginine comprising culturing a coryneform bacterium, which has L-arginine producing ability and has been transformed with a polynucleotide that encodes the LysE protein having the amino acid sequence of SEQ ID NO:
- 26, in a medium to produce and accumulate L-arginine in the medium, and collecting the L-arginine from the medium, wherein said coryneform bacterium comprises a polynucleotide that encodes an arginine repressor having the amino acid sequence of SEQ ID NO:
- 18 which has been disrupted and overproduces L-arginine as compared to the unmodified coryneform bacterium.
A method for producing L-arginine comprising culturing a coryneform bacterium, which has L-arginine producing ability and has been transformed with a polynucleotide that encodes the LysE protein having the amino acid sequence of SEQ ID NO:
- 26, in a medium to produce and accumulate L-arginine in the medium, and collecting the L-arginine from the medium, wherein intracellular activity of enzymes in the L-arginine biosynthetic pathway comprising argCJBDFGH products has been enhanced in said coryneform bacterium and said coryneform bacterium overproduces L-arginine as compared to the unmodified coryneform bacterium.
Dependencies are inferred from the claim text.