EG307 polynucleotides and uses thereof
AssigneeEvolutionary Genomics, Inc.
InventorWalter Messier
Life of this patent
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Abstract
The present invention provides methods for identifying polynucleotide and polypeptide sequences which may be associated with commercially or aesthetically relevant traits in domesticated plants or animals. The methods employ comparison of homologous genes from the domesticated organism and its ancestor to identify evolutionarily significant changes and evolutionarily neutral changes. Sequences thus identified may be useful in enhancing commercially or aesthetically desirable traits in domesticated organisms or their wild ancestors.
Claims12
Plant cells, comprising heterologous DNA encoding an EG307 polypeptide, wherein said polypeptide is selected from the group consisting of:
- a) a polypeptide encoded by a polynucleotide selected from the group consisting of SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:91, SEQ ID NO:33, SEQ ID NO:34, and SEQ ID NO:35;
- b) a polypeptide encoded by a polynucleotide having at least 95% sequence identity to a polynucleotide in a), wherein the presence of said polynucleotide is a marker of increased yield in a plant of the genus Oryza or Zea;
- c) a polypeptide comprising SEQ ID NO:6 or SEQ ID NO:36;
- and
- d) a polypeptide having at least 95% sequence identity to a polypeptide of c), wherein the presence of a polynucleotide encoding a polypeptide of (d) is a marker of increased yield in a plant of the genus Oryza or Zea.
A transgenic plant containing heterologous DNA which encodes an EG307 polypeptide that is expressed in plant tissue, and said polypeptide is selected from the group consisting of:
- a) a polypeptide encoded by a polynucleotide selected from the group consisting of SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:91, SEQ ID NO:33, SEQ ID NO:34, and SEQ ID NO:35;
- b) a polypeptide encoded by a polynucleotide having at least 95% sequence identity to a polynucleotide in a), wherein the presence of said polynucleotide is a marker of increased yield in a plant of the genus Oryza or Zea;
- c) a polypeptide comprising SEQ ID NO:6 or SEQ ID NO:36;
- and
- d) a polypeptide having at least 95% sequence identity to a polypeptide of c), wherein the presence of a polynucleotide encoding a polypeptide of (d) is a marker of increased yield in a plant of the genus Oryza or Zea.
An isolated polynucleotide which includes a promoter operably linked to a polynucleotide that encodes an EG307 gene in plant tissue, said polynucleotide selected from the group consisting of:
- a) a polynucleotide selected from the group consisting of SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:91, SEQ ID NO:33, SEQ ID NO:34, and SEQ ID NO:35;
- b) a polynucleotide having at least 95% sequence identity to a polynucleotide of a), wherein the presence of said polynucleotide is a marker of increased yield in a plant of the genus Oryza or Zea;
- c) a polynucleotide encoding a polypeptide comprising SEQ ID NO:6 or SEQ ID NO:36;
- and
- d) a polynucleotide encoding a polypeptide having at least 95% sequence identity to SEQ ID NO:6 or SEQ ID NO:36, wherein the presence of a polynucleotide encoding a polypeptide of (d) is a marker of increased yield in a plant of the genus Oryza or Zea.
A transfected host cell comprising a host cell transfected with a construct comprising a promoter, enhancer or intron polynucleotide from an evolutionarily significant EG307 polynucleotide or any combination thereof, operably linked to a polynucleotide encoding a reporter protein, wherein said EG307 polynucleotide is capable of increasing the yield of a plant, wherein said EG307 polynucleotide is selected from the group consisting of:
- a) a polynucleotide selected from the group consisting of SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:91, SEQ ID NO:33, SEQ ID NO:34, and SEQ ID NO:35;
- b) a polynucleotide having at least 95% sequence identity to a polynucleotide of a), wherein the presence of said polynucleotide is a marker of increased yield in a plant of the genus Oryza or Zea;
- c) a polynucleotide encoding a polypeptide comprising SEQ ID NO:6 or SEQ ID NO:36;
- and
- d) a polynucleotide encoding a polypeptide having at least 95% sequence identity to SEQ ID NO:6 or SEQ ID NO:36, wherein the presence of a polynucleotide encoding a polypeptide of (d) is a marker of increased yield in a plant of the genus Oryza or Zea.
A method of identifying an agent which may modulate yield, said method comprising contacting at least one candidate agent which may modulate function of an EG307 polynucleotide or polypeptide with a plant or cell comprising an EG307 gene, wherein the agent is identified by its ability to modulate yield, and wherein said EG307 gene comprises a polynucleotide selected from the group consisting of:
- a) a polynucleotide selected from the group consisting of SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:91, SEQ ID NO:33, SEQ ID NO:34, and SEQ ID NO:35;
- b) a polynucleotide having at least 95% sequence identity to a polynucleotide of a), wherein the presence of said polynucleotide is a marker of increased yield in a plant of the genus Oryza or Zea;
- c) a polynucleotide encoding a polypeptide comprising SEQ ID NO:6 or SEQ ID NO:36;
- and
- d) a polynucleotide encoding a polypeptide having at least 95% sequence identity to SEQ ID NO:6 or SEQ ID NO:36, wherein the presence of a polynucleotide encoding a polypeptide of (d) is a marker of increased yield in a plant of the genus Oryza or Zea.
A method of producing an EG307 polypeptide comprising:
- a) providing a cell transfected with a polynucleotide encoding an EG307 polypeptide positioned for expression in the cell;
- b) culturing the transfected cell under conditions for expressing the polynucleotide;
- and
- c) isolating the EG307 polypeptide, wherein said polypeptide is selected from the group consisting of:
- i) a polypeptide encoded by a polynucleotide selected from the group consisting of SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:91, SEQ ID NO:33, SEQ ID NO:34, and SEQ ID NO:35;
- ii) a polypeptide encoded by a polynucleotide having at least 95% sequence identity to a polynucleotide in i), wherein the presence of said polynucleotide is a marker of increased yield in a plant of the genus Oryza or Zea;
- iii) a polypeptide comprising SEQ ID NO:6 or SEQ ID NO:36;
- and
- iv) a polypeptide having at least 95% sequence identity to a polypeptide of iii), wherein the presence of a polynucleotide encoding a polypeptide of (d) is a marker of increased yield in a plant of the genus Oryza or Zea.
Dependencies are inferred from the claim text.