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US 7,252,961 B2Granted
Competitive immunoassay using complexed analyte derivatives
AssigneeDade Behring Marburg GmbH
InventorsStephan Neuenhofer, Heinz-Jürgen Skrzipczyk, Peter Molz, Reinhard Käsmarker
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Abstract
The invention relates to a method for the detection of an analyte in a competitive immunoassay in the presence of an analyte derivative and first and second receptor molecules, and to a kit for carrying out this detection method.
Claims22
A method for determining the presence or amount of an analyte in a sample in a competitive immunoassay, which comprises the following steps:
- (a) incubating an analyte derivative with the sample, a first receptor molecule which is specific for the analyte and the analyte derivative, and a second receptor molecule which is specific for the analyte derivative and the analyte, wherein the analyte derivative is immobilized on a solid phase;
- (b) binding the analyte derivative with the first receptor molecule, wherein the first receptor molecule is labeled with a label, and wherein the second receptor molecule masks the analyte derivative;
- (c) separating the first receptor molecule not bound to the analyte derivative;
- and
- (d) detecting the first receptor molecule bound to the analyte derivative and determining the presence or amount of the analyte in the sample.
2 more elements
A method for determining the presence or amount of an analyte in a sample in a competitive immunoassay, which comprises the following steps:
- (a) incubating an analyte derivative with the sample, a first receptor molecule which is specific for the analyte and the analyte derivative, and a second receptor molecule which is specific for the analyte derivative and the analyte, wherein the first receptor molecule is immobilized on a solid phase;
- (b) binding the analyte derivative with the first receptor molecule, wherein the analyte derivative is labeled with a label, and wherein the second receptor molecule masks the analyte derivative;
- (c) separating the analyte derivative not bound to the first receptor molecule;
- and
- (d) detecting the analyte derivative bound to the first receptor molecule and determining the presence or amount of the analyte in the sample.
2 more elements
Dependencies are inferred from the claim text.