Assays for diagnosis of thrombophilic disease
AssigneeMount Sinai School of Medicine of the City University of New York
InventorJacob H. Rand
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Abstract
The present invention provides methods for diagnosing and/or monitoring thrombophilic disease in a patient that can result from the antiphospholipid antibody syndrome (aPL syndrome). The methods of the invention are premised on the inhibition of binding of an anticoagulant protein, annexin, preferably annexin-V, to phospholipids by antiphospholipid (aPL) antibodies in a patient blood sample.
Claims9
A method of diagnosing and/or monitoring antiphospholipid antibody syndrome in a patient comprising
- a. incubating in duplicate a phospholipid dependent coagulation test reagent with a sample of anticoagulated patient plasma,
- b. calcifying the plasma in one of the duplicate samples of step a in the presence of a standardized amount of annexin V and calcifying the plasma in the other sample in the absence of annexin V, and
- c. monitoring the time until clot formation occurs in each sample, and
- d. comparing the time until clot formation in the sample in the presence of annexin V to the sample in the absence of annexin V, wherein a reduced anticoagulant effect in the presence of annexin V indicates antiphospholipid antibody syndrome in the patient.
A method of diagnosing and/or monitoring antiphospholipid antibody syndrome in a patient comprising
- a. incubating in duplicate a phospholipid dependent coagulation test reagent with a sample of anticoagulated patient plasma or isolated patient IgG,
- b. removing unbound plasma or IgG from the coagulation test reagent,
- c. incubating the duplicate samples of step b with a sample of control plasma,
- d. calcifying the control plasma in one of the duplicate samples of step c in the presence of a standardized amount of annexin V and calcifying the control plasma in the other sample in the absence of annexin V and
- e. monitoring the time until clot formation occurs in each sample, and
- f. comparing the time until clot formation in the sample in the presence of annexin V to the sample in the absence of annexin V wherein a reduced anticoagulant effect in the presence of annexin V indicates antiphospholipid antibody syndrome disease in the patient.
Dependencies are inferred from the claim text.