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AssigneeFlanders Interuniversity Institute for Biotechnology
InventorsRoland Contreras, Nico L. M. Callewaert, Steven C. J. Geysens, Vladimir Kaigorodov, Vervecken Wouter
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The present invention provides genetically engineered strains of methylotrophic yeast including Pichia and especially Pichia pastoris capable of producing proteins with reduced or modified glycosylation. Methods of producing glycoproteins with reduced and/or modified glycosylation using such genetically engineered strains of Pichia are also provided. Vectors, which comprise coding sequences for α-1,2-mannosidase I, glucosidase II, GlcNAc-tranferase I and mannosidase II or comprising OCH1 disrupting sequence, for transforming methylotrophic yeasts are contemplated by the present invention. Kit for providing the comtemplated vectors are also included in this invention.
A methylotrophic yeast strain, transformed with a nucleotide sequence coding for a protein comprising the functional part of human GlcNAc-transferase I encoded by SEQ ID NO:
A kit for modification of protein glycosylation in a methylotrophic yeast strain, comprising a vector which comprises a nucleotide sequence coding for a protein comprising the functional part of human GlcNAc-transferase I encoded by SEQ ID NO:
Dependencies are inferred from the claim text.