US 7,252,929 B2 · Methods for identifying alpha PDGFR agonists and antagonists

Abstract

Discoveries are disclosed that show particular aspects of recombinant DNA technology can be used successfully to produce a hitherto unknown type of human Platelet-Derived Growth Factor (PDGF) receptor protein free of other PDGF receptors. These proteins can be produced from DNA segments in cells in various functional forms. These forms variously enable biochemical and functional studies of these novel receptors as well as production of antibodies. Means are described for determining the level of expression of genes for specific types of PDGF receptor proteins, for example, by measuring mRNA in cells with PDGF receptor type-specific DNA probes or by measuring antigen in biological samples with type-specific antibodies.

Claims7

Dependent claims

A method of determining the ability of a compound to bind to Platelet-derived growth factor receptor alpha (α PDGFR) comprising:

a) providing a sample of cells having a recombinant construct expressing α PDGFR;
b) exposing said sample of cells expressing α PDGFR to a compound suspected of binding to α PDGFR;
and
c) measuring the amount of α PDGFR activity after exposure of the cell sample.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

10/700,249Filed 3 Nov 2003 · continuation

Priority

9 Feb 1989

Published

US 2005/0112582 A126 May 2005

Granted

7 Aug 2007

Term ends

Not estimatedThe source records don’t support an estimate.

Classification11 codes

G01N 33/74
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing:…
C07K 14/71
Receptors; Cell surface antigens; Cell surface determinants: for growth factors; for growth regulators
C07K 16/22
Against material from animals or humans: against growth factors ; against growth regulators
C07K 16/2863
Against receptors, cell surface antigens or cell surface determinants: against receptors for growth factors, growth regulators

Family

Application07/308,282Parent of this continuation · filed 9 Feb 1989

Application07/915,884Parent of this continuation · filed 20 Jul 1992

Application08/439,095Parent of this division · filed 11 May 1995

Application08/460,656Parent of this division · filed 2 Jun 1995

Application09/769,987Parent of this continuation · filed 25 Jan 2001

US 6,228,600 B1Parent of this division · filed 8 May 2001

US 2005/0112582 A1Pre-grant publication · published 26 May 2005

US 6,660,488 B2Parent of this continuation

Application09/769,987Parent of this division

Application08/439,095Parent of this continuation

US 2002/0055129 A1Published application

US 7,427,671 B1Related patent

US 7,252,929 B2This patent

From the US filing history. Foreign family members aren’t listed.

Methods for identifying alpha PDGFR agonists and antagonists

Life of this patent

Methods for identifying alpha PDGFR agonists and antagonists

Life of this patent

Abstract

Claims7