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AssigneeAtheroGenics, Inc.
InventorsCharles Kunsch, Signe E. Varner, Xilin Chen, Jayraz Luchoomun
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This invention is in the area of regulatory DNA sequences and their methods of use. Specifically, DNA sequences found in the regulatory region of cytoprotective genes are described that are termed cytoprotective response elements. DNA constructs also are provided that include cytoprotective response elements operably linked to heterologous protein coding sequence, as well as cells and non-human organisms that include the cytoprotective response elements optionally operably linked to a heterologous protein coding sequence, and a method for screening for a compound that increases mRNA or protein regulated by a cytoprotective response element. It has been discovered that the cytoprotective response elements mediate the coordinate activation of certain genes that protect cells from damaging effects of oxidative stress, and that they do so, for example during conditions of hemodynamic shear stress.
An isolated nucleic acid consisting of SEQ ID NO:1.
A nucleic acid construct consisting of SEQ ID NO:1, operably linked to a heterologous protein coding sequence.
A nucleic acid construct comprising a cytoprotective response element consisting of SEQ ID NO:1.
A nucleic acid construct comprising a cytoprotective response element consisting of SEQ ID NO:1 operably linked to a promoter.
A nucleic acid construct consisting of one to ten cytoprotective response elements wherein the cytoprotective response element is SEQ ID NO:1 that induces the expression of microsomal epoxide hydroxylase.
An isolated nucleic acid construct comprising two to ten cytoprotective response elements wherein the cytoprotective response element is SEQ ID NO:1.
An isolated nucleic acid construct comprising three to ten cytoprotective response elements wherein the cytoprotective response element is SEQ ID NO:1.
Dependencies are inferred from the claim text.