Complex of hepatitis B virus-specific antigenic peptides associated with heat shock proteins and the application thereof
AssigneeInstitute of Microbiology, Chinese Academy of Sciences
InventorsBo Tian, Songdong Meng, George F. Gao
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Abstract
The present invention provides a kind of HBV-antigen bound to heat shock proteins (hsps) which comprises core antigen, surface antigen and polymerase antigen. The present invention also provides a complex of HBV antigen bound to heat shock protein gp96 and hsp78, as well as a method for preparing the complex. The complex includes a complex of gp96 and hsp78 non-covalently bound to antigenic polypeptide, as well as a fusion protein of both which results from covalent binding. Such complex can be used to prepare therapeutic vaccine for treating hepatitis B and primary hepatocellular carcinoma.
Claims9
A composition, comprising a complex of HBV antigen and a human heat shock protein gp96, wherein the HBV antigen consists of the amino acid sequence of:
- YVNTNMG (SEQ ID NO:
- 1).
A composition comprising complexes of (i) a human heat shock protein (HSP) molecule gp96, and (ii) variants of hepatitis B virus (HBV) antigen, wherein the HSP-HBV antigen complexes, when administered to an individual, induce or enhance an immune response against HBV infection, wherein the variants of HBV antigen are:
- SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, and SEQ ID NO:
- 10.
A composition comprising HBV antigens, wherein the antigens are:
- SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, and SEQ ID NO:
- 10.
Dependencies are inferred from the claim text.