US 7,247,619 B2 · Viral vectors useful in induction of humoral or cellular immunity

Abstract

The invention features recombinant viral vectors that take advantage of the activity of picornaviral protein 3A in modulating cytokine secretion and antigen presentation on MHC Class I (MHC I), which in turn provides for modulation of a Th1-mediated immune response to the transfected host cell. Specifically, a recombinant viral vector comprising a sequence encoding picornaviral protein 3A provides for decreased antigen presentation on MHC I and a decreased incidence of Th1-mediated immune response, while a recombinant picornaviral vector that is deficient in protein 3A production provides for increased cytokine secretion, increased antigen presentation on MHC I and increased Th1-mediated immune response towards the transfected host cell. The invention also features methods of inducing humoral or cellular immunity using the vectors.

Claims16

Dependent claims

A recombinant viral vector comprising:

a first nucleic acid comprising a sequence encoding an exogenous polypeptide for expression in a host cell;
a second nucleic acid comprising a sequence encoding a picornaviral protein 3A for expression in the host cell, wherein the picornaviral 3A protein is selected from a poliovirus 3A protein, a coxsackievirus B3 protein, and a rhinovirus 14 protein;
wherein the viral vector is a recombinant retroviral vector, a recombinant adenoviral vector, a recombinant alphavirus vector, a recombinant adeno-associated viral vector, or a recombinant poxvirus vector and wherein expression of the recombinant picornaviral protein 3A results in inhibition of presentation of the exogenous polypeptide on an MHC class I molecule of the host cell.

Dependencies are inferred from the claim text.

Viral vectors useful in induction of humoral or cellular immunity

Life of this patent

Viral vectors useful in induction of humoral or cellular immunity

Life of this patent

Abstract

Claims16