Testing multiple fluid samples with multiple biopolymer arrays
AssigneeAgilent Technologies, Inc.
InventorsSueAnn C. Dahm, Arthur Schleifer, Carol T. Schembri, Douglas A. Amorese
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Abstract
A method of testing multiple fluid samples with multiple biopolymer arrays. A cover is assembled to a contiguous substrate carrying on a first side, multiple arrays each with multiple regions of biopolymers linked to the substrate, such that the cover and the substrate together form a plurality of chambers each containing a biopolymer array and each being accessible through its own port. Multiple fluid samples are introduced into respective chambers through a port of each such that the fluid samples contact respective arrays. A binding pattern of the arrays is observed. An apparatus and kit useful in such methods, are also provided.
Claims20
A method of testing multiple fluid samples, said method comprising:
- (a) providing a substrate carrying multiple arrays on a first side of said substrate where each array comprises multiple regions of biopolymers linked to said substrate in a predetermined configuration;
- (b) assembling a cover to the first side of the substrate such that the cover and the substrate together form a plurality of chambers each containing a biopolymer array and each being accessible through its own first and second ports positioned on the same side of said substrate;
- (b) introducing multiple test samples and a reference sample into respective chambers through a port of each chamber such that the fluid samples contact respective arrays, wherein at least one of the arrays is exposed to a test sample under a first set of conditions and at least another one of the arrays is exposed to a reference sample but not test sample under the same set of conditions;
- and
- (c) observing a binding pattern of the arrays.
A method of testing multiple fluid samples, said method comprising:
- (a) providing a substrate carrying multiple arrays on a first side of said substrate where each array comprises multiple regions of nucleic acids linked to said substrate in a predetermined configuration;
- (b) assembling a cover to the first side of the substrate such that the cover and the substrate together form a plurality of chambers each containing a biopolymer array and each being accessible through its own first and second ports positioned on the same side of said substrate;
- (b) introducing the multiple test samples and reference sample into respective chambers through a port of each chamber such that the fluid samples contact respective arrays, wherein at least one of the arrays is exposed to a test sample under a first set of conditions and at least another one of the arrays is exposed to a reference sample but not test sample under the same set of conditions;
- and
- (c) observing a binding pattern of the arrays.
A method of testing multiple fluid samples, said method comprising:
- (a) providing a planar substrate carrying multiple arrays on a first side of said planar substrate where each array comprises multiple regions of biopolymers linked to said substrate in a predetermined configuration;
- (b) assembling a cover to the first side of the substrate such that the cover and the substrate together form a plurality of chambers each containing a biopolymer array and each being accessible through its own port positioned on the same side of said substrate;
- (b) introducing multiple test samples and reference sample into respective chambers through a port of each chamber such that the fluid samples contact respective arrays, wherein at least one of the arrays is exposed to a test sample under a first set of conditions and at least another one of the arrays is exposed to a reference sample but not test sample under the same set of conditions;
- and
- (c) observing a binding pattern of the arrays.
Dependencies are inferred from the claim text.