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US 7,247,488 B2Granted
Method and kit for testing a multi-channel blood assay cartridge
AssigneeMedtronic, Inc.
InventorsJyotsna Ghai, Mark A. Thompson, Colleen Lutz, Narayanan Ramamurthy, Charlene X. Yuan
Life of this patent
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Abstract
A method and kit for testing a multi-channel blood-testing cartridge. In particular, blood-testing cartridges are tested with plasma samples with clotting times measured to indicate whether a batch of cartridges is suitable for testing the blood of a patient.
Claims21
A method comprising the steps of:
- selecting a first test cartridge having a first channel with an anticoagulant/antithrombotic as a reagent therein and a second channel without the anticoagulant/antithrombotic as a reagent therein;
- preparing a first plasma sample that has a clotting time that may be altered by the presence of the anticoagulant/antithrombotic reagent;
- adding a first portion of the first plasma sample to the first channel of the first cartridge;
- adding a second portion of the first plasma sample to the second channel of the first cartridge;
- determining the time required for a clot to be formed in each of the first and second channels for the first plasma sample;
- preparing a second plasma sample that includes an agent capable of neutralizing the anticoagulant/antithrombotic reagent;
- selecting a second test cartridge having a first channel with the anticoagulant/antithrombotic as a reagent therein and a second channel without an anticoagulant/antithrombotic as a reagent therein;
- adding a first portion of the second plasma sample to the first channel;
- adding a second portion of the second plasma sample to the second channel;
- and
- determining the time required for a clot to be formed in each of the first and second channels for the second plasma sample, wherein the anticoagulant/antithrombotic reagent is heparin.
8 more elements
A method comprising the steps of:
- selecting a first test cartridge having a first channel with a anticoagulant/antithrombotic as a reagent therein;
- a second channel with the anticoagulant/antithrombotic as a reagent therein and also with a blood factor as a reagent therein that is responsive to the anticoagulant/antithrombotic;
- and
- a third channel without the anticoagulant/antithrombotic as a reagent therein and also without the blood factor as a reagent therein;
- preparing a first plasma sample that has a clotting time that may be altered by the presence of the anticoagulant/antithrombotic but is deficient in the blood factor;
- adding a first portion of the first plasma sample to the first channel of the first cartridge;
- adding a second portion of the first plasma sample to the second channel of the first cartridge;
- adding a third portion of the first plasma sample to the third channel of the first cartridge;
- determining the time required for a clot to be formed in each of the first, second and third channels for the first plasma sample;
- preparing a second plasma sample that comprises a neutralizing agent for the anticoagulant/antithrombotic;
- selecting a second test cartridge having a first channel with the anticoagulant/antithrombotic as a reagent therein, a second channel with the anticoagulant/antithrombotic as a reagent therein and also with the blood factor therein, and a third channel without the anticoagulant/antithrombotic as a reagent therein and also without the blood factor as a reagent therein;
- adding a first portion of the second plasma sample to the first channel;
- adding a second portion of the second plasma sample to the second channel;
- adding a third portion of the second plasma sample to the third channel;
- determining the time required for a clot to be formed in each of the first, second and third channels for the second plasma sample, wherein the anticoagulant/antithrombotic reagent is heparin and the blood factor is antithrombin III (ATIII).
12 more elements
A quality test kit comprising:
- (1) a first plasma sample that has a clotting time that may be altered by the presence of heparin but is deficient in antithrombin III (AT III);
- (2) a second plasma sample that includes an agent capable of neutralizing the heparin and
- (3) a first and second test cartridge having a first channel with heparin as a reagent therein and a second channel without heparin therein.
Dependencies are inferred from the claim text.