Simian adenovirus nucleic acid and amino acid sequences, vectors containing same, and methods of use
AssigneeThe Trustees of the University of Pennsylavania
InventorsJames M. Wilson, Guangping Gao, Soumitra Roy
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Abstract
A recombinant vector comprising capsid proteins derived from simian adenovirus Pan5, Pan6, Pan7, SV1, SV25 or SV39 sequences are described. These vectors contain a heterologous gene under the control of regulatory sequences packaged in the capsid. A cell line which expresses simian adenovirus gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.
Claims13
An adenovirus having an artificial adenovirus capsid comprising a hexon protein of Pan7 having the sequence of SEQ ID NO:11 and a heterologous adenovirus capsid protein sequence.
A recombinant adenovirus having a capsid comprising an AdPan7 hexon protein having the amino acid sequence of SEQ ID NO:11 and a nucleic acid sequence heterologous to AdPan7.
A recombinant adenovirus having a capsid comprising a hexon containing a fragment of the AdPan7 hexon protein and a nucleic acid sequence heterologous to the AdPan7, wherein the fragment of the AdPan7 hexon protein is the AdPan7 hexon protein of SEQ ID NO:11 with an N-terminal or C-terminal truncation of about 50 amino acids or is selected from the group consisting of:
- amino acid residues 125 to 443 of SEQ ID NO:
- 11;
- amino acid residues 138 to 441 of SEQ ID NO:
- 11;
- amino acid residues 138 to 163 of SEQ ID NO:
- 11;
- amino acid residues 170 to 176 of SEQ ID NO:
- 11;
- and
- amino acid residues 404 to 430 of SEQ ID NO:
- 11.
An adenovirus having a capsid comprising an AdPan7 hexon protein having the amino acid sequence of SEQ ID NO:11, said capsid encapsidating a heterologous nucleic acid sequence operably linked to expression control sequences which direct transcription, translation, and/or expression thereof in a host cell.
Dependencies are inferred from the claim text.