Non-specific binding resistant protein arrays and methods for making the same
AssigneeZyomyx, Inc.
InventorsPeter Wagner, Peter Kernen, Hongbo Lu, Huu Tran
Life of this patent
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Abstract
Arrays of protein-capture agents useful for the simultaneous detection of a plurality of proteins which are the expression products, or fragments thereof, of a cell or population of cells in an organism are provided. A variety of antibody arrays, in particular, are described. Methods of both making and using the arrays of protein-capture agents are also disclosed. The invention arrays are particularly useful for various proteomics applications including assessing patterns of protein expression and modification in cells.
Claims27
A molecular array for characterizing molecular interaction events, comprising:
- (a) a substrate;
- and
- (b) at least one discrete molecular deposition domain on said substrate wherein the spatial address of the domain is less than one micron squared in area and each domain includes a biomolecule deposited on the substrate at a pre-selected location.
A molecular array for characterizing molecular interaction events, comprising:
- (a) a substrate;
- and
- (b) at least one discrete molecular deposition domain on said substrate wherein the spatial address of the domain is less than one micron squared in area and each domain includes a silane deposited on the substrate at a pre-selected location.
An array for the identification of a target material comprising:
- a substrate including a substantially flat surface;
- and
- an at least one discrete deposition domain deposited on said surface, said deposition domain being smaller than one micron squared in total area and deposited at a pre-selected location on the surface, the deposition domain including a long chain biomolecular deposition material having the capacity to bind the target material.
An array of deposition domains for the detection of one or more pre-determined target materials comprising:
- a solid glass substrate including a substantially flat surface;
- and
- an at least one discrete domain deposited on the surface of the substrate, each domain being deposited at a known location and being smaller than one micron squared in area, each domain further including at least one type of molecule with a binding affinity for one or more of the target materials, at least two domains containing different biologically or chemically based molecules.
A molecular array for characterizing molecular interaction events, comprising:
- (a) a substrate;
- and
- (b) at least one molecular deposition domain on said substrate wherein the spatial address of the domain is less than one micron squared in area, each domain includes a biologically or chemically based molecule directly deposited on the substrate at a pre-selected location, at least two domains containing different biologically or chemically based molecules.
An array, comprising:
- (a) a substrate;
- and
- (b) at least one patch immobilized on the substrate at a pre-selected location, the at least one patch having an area of about 1 micron squared.
Dependencies are inferred from the claim text.