Broad host range pBBR1-based plasmid mutant derivatives having altered plasmid copy number
AssigneeE. I. Du Pont de Nemours and Company
InventorsQiong Cheng, Luan Tao
Life of this patent
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Abstract
The present invention describes a mutant plasmid replication control region having the ability to convey a phenotype of altered plasmid copy number to the plasmid on which it resides. The mutant replication control region is based on a similar region isolated from the pBBR1 plasmid family. Plasmids containing this replication control region cannot be classed as belonging to any known incompatibility group and thus may co-exist with a broad range of other plasmids in a single host.
Claims15
A mutant replication control region having a nucleotide sequence as set forth in nucleotides 2478–3765 of SEQ ID NO:1 and having one point mutation independently selected from the group consisting of:
- a) a mutation of G to A at nucleotide 2490;
- b) a mutation of C to Tat nucleotide 2496;
- c) a mutation of T to C at nucleotide 2579;
- d) a mutation by deletion of C at nucleotide 2633;
- e) a mutation by deletion of C at nucleotide 2634;
- f) a mutation of T to C at nucleotide 2663;
- g) a mutation of A to G at nucleotide 2771;
- h) a mutation of T to C at nucleotide 2805;
- i) a mutation of C to A at nucleotide 2838;
- j) a mutation of T to C at nucleotide 2914;
- k) a mutation of T to C at nucleotide 2935;
- l) a mutation of C to T at nucleotide 3003;
- m) a mutation for substitution of G to A at nucleotide 3165;
- n) a mutation of T to G at nucleotide 3262;
- o) a mutation of C to T at nucleotide 3269;
- p) a mutation of T to C at nucleotide 3344;
- q) a mutation of C to T at nucleotide 3347;
- r) a mutation for substitution of C to A at nucleotide 3456;
- s) a mutation for substitution of T to C at nucleotide 3468;
- t) a mutation for substitution of A to G at nucleotide 3570;
- u) a mutation of T to C at nucleotide 3604;
- v) a mutation of A to C at nucleotide 3641;
- w) a mutation of A to G at nucleotide 3729;
- x) a mutation of T to A at nucleotide 3739;
- and
- y) a mutation of T to C at nucleotide 3747.
A mutant replication gene having the nucleotide sequence as set forth in SEQ ID NO:3 wherein:
- a) nucleotide position 117 is A;
- b) nucleotide position 214 is G;
- c) nucleotide position 221 is T;
- d) nucleotide position 296 is C;
- e) nucleotide position 299 is T;
- f) nucleotide position 408 is A;
- g) nucleotide position 420 is C;
- h) nucleotide position 522 is G;
- i) nucleotide position 556 is C;
- and
- j) nucleotide position 593 is C.
A mutant replication gene as set forth as nucleotides 3049–3711 of SEQ ID NO:1 comprising one point mutation independently selected from the group consisting of:
- a) a mutation for substitution of G to A at nucleotide 117;
- b) a mutation of T to G at nucleotide 214;
- c) a mutation of C to T at nucleotide 221;
- d) a mutation of T to C at nucleotide 296;
- e) a mutation of C to T at nucleotide 299;
- l) a mutation for substitution of G to A at nucleotide 408;
- g) a mutation for substitution of T to C at nucleotide 420;
- h) a mutation for substitution of A to G at nucleotide 522;
- i) a mutation of T to C at nucleotide 556;
- and
- j) a mutation of A to C at nucleotide 593.
A mutant replication control region selected from the group consisting of SEQ ID NOs:6, 8, 10, 12, 13, 14, 15, 17, 18, 20, and 21.
Dependencies are inferred from the claim text.